What Is HPLC Test In Pregnancy?

Charlotte Miller

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Are you curious to know what is HPLC test in pregnancy? You have come to the right place as I am going to tell you everything about HPLC test in pregnancy in a very simple explanation. Without further discussion let’s begin to know what is HPLC test in pregnancy?

Pregnancy is a time of profound physical and hormonal changes in a woman’s body. Ensuring the well-being of both the mother and the developing fetus is paramount. High-Performance Liquid Chromatography (HPLC) is a diagnostic test that plays a crucial role in monitoring pregnancy-related conditions and ensuring a healthy pregnancy. In this blog, we will explore what the HPLC test in pregnancy is, its significance, and the conditions it helps diagnose and manage.

What Is HPLC Test In Pregnancy?

HPLC, short for High-Performance Liquid Chromatography, is an advanced laboratory technique used to separate, identify, and quantify components of complex mixtures. In the context of pregnancy, HPLC is employed to analyze blood samples to measure specific substances and markers related to maternal and fetal health.

Significance Of HPLC In Pregnancy

The HPLC test in pregnancy serves multiple purposes:

  1. Monitoring Diabetes: One of the primary applications of HPLC during pregnancy is the monitoring of gestational diabetes. This condition occurs when the body cannot produce enough insulin to meet the increased demands during pregnancy. HPLC is used to measure glucose levels accurately and adjust treatment plans accordingly.
  2. Detecting Anemia: HPLC can be used to assess hemoglobin variants and diagnose different types of anemia. Anemia during pregnancy can pose risks to both the mother and the baby, making early detection and management crucial.
  3. Identifying Hemoglobinopathies: Hemoglobinopathies are inherited blood disorders, such as sickle cell disease and thalassemia. HPLC can identify specific hemoglobin variants and help assess the risk of these conditions in the fetus.
  4. Monitoring Medication Levels: Pregnant women with pre-existing medical conditions may require specific medications. HPLC can be used to monitor drug levels in the bloodstream, ensuring that the dosage remains within the therapeutic range and is safe for both the mother and the baby.
  5. Screening for Preeclampsia: Preeclampsia is a serious pregnancy complication characterized by high blood pressure and damage to organs such as the liver and kidneys. HPLC can aid in identifying biomarkers associated with preeclampsia, enabling early diagnosis and intervention.
  6. Assessing Fetal Health: In some cases, HPLC can be used to assess the health of the fetus. For example, it can measure the levels of bilirubin in the amniotic fluid to identify conditions like hemolytic disease of the newborn.

How The HPLC Test Works?

The HPLC test involves the following steps:

  1. Sample Collection: A blood sample is collected from the pregnant woman, usually through a vein in the arm.
  2. Sample Preparation: The blood sample is prepared for analysis, which may include separating plasma or serum from the whole blood.
  3. Chromatographic Separation: The prepared sample is injected into an HPLC machine, which uses high pressure to push the sample through a chromatographic column. Different components in the sample move at different speeds and are separated based on their interactions with the column material.
  4. Detection: As the separated components exit the column, they are detected by a detector, typically based on their absorption of ultraviolet (UV) light. The detector generates signals that are converted into quantitative data.
  5. Data Analysis: The data collected from the HPLC analysis is processed and interpreted to determine the levels of specific substances or markers in the blood sample.


The HPLC test in pregnancy is a valuable diagnostic tool that contributes to the well-being of both expectant mothers and their developing babies. It enables healthcare providers to monitor and manage pregnancy-related conditions such as gestational diabetes, anemia, hemoglobinopathies, and preeclampsia, ultimately ensuring a safer and healthier pregnancy journey for women and their families.


What Is The HPLC Blood Test For Pregnancy?

It is a test to look for thalassemia and other haemoglobinopathies in parents during their pregnancy.

What Is The Normal Range For HPLC In Pregnancy?

The normal range of hemoglobin in men is between 13.5 g/dL and 17.5 g/dL and the optimum range of hemoglobin in non-pregnant females is between 12.0 g/dL and 15.5 g/dL and in pregnant females is 11 g/dL and 16 g/dL.

What Is The Purpose Of HPLC Test?

HPLC is a sensitive and precise method for the identification of Hb A2, Hb F and abnormal haemoglobins. It has become the method of choice for thalassaemia screening because of its speed and reliability.

What Are The Symptoms Of HPLC Test?

Although many do not result in clinical or hematologic effects, clinical symptoms that can be associated with hemoglobin disorders include microcytosis, sickling disorders, hemolysis, erythrocytosis/polycythemia, cyanosis/hypoxia, anemia (chronic, compensated or episodic), and increased methemoglobin or sulfhemoglobin …

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